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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using NEURL Polyclonal antibody (18898-1-AP)

    WB analysis of mouse brain using 18898-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NEURL Polyclonal antibody (18898-1-AP)

    WB analysis using 18898-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NEURL Polyclonal antibody (18898-1-AP)

    WB analysis of MCF-7 using 18898-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using NEURL Polyclonal antibody (18898-1-AP)

    WB analysis of Y79 using 18898-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 18898-1-AP (NEURL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NEURL Polyclonal antibody (18898-1-AP)

    IP experiment of mouse brain using 18898-1-AP

    IP result of anti-NEURL (IP:18898-1-AP, 4ug; Detection:18898-1-AP 1:600) with mouse brain tissue lysate 2560ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NEURL Polyclonal antibody (18898-1-AP)

    IHC staining of mouse brain using 18898-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18898-1-AP (NEURL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NEURL Polyclonal antibody (18898-1-AP)

    IHC staining of mouse brain using 18898-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18898-1-AP (NEURL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using NEURL Polyclonal antibody (18898-1-AP)

    IF Staining of MCF-7 using 18898-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using NEURL antibody (18898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  • Featured Product
  • KD/KO Validated

NEURL Polyclonal antibody

NEURL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18898-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

neu, h-neuralized 1, h neuralized 1, h neu, EC:2.3.2.27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, mouse brain tissue, Y79 cells, SH-SY5Y cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18898-1-AP targets NEURL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NEURL fusion protein Ag13531 相同性解析による交差性が予測される生物種
Full Name neuralized homolog (Drosophila)
Calculated molecular weight 59 kDa
Observed molecular weight 60-62 kDa
GenBank accession numberBC026336
Gene Symbol NEURL
Gene ID (NCBI) 9148
RRIDAB_10642557
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76050
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEURL(Neuralized-like protein 1A) is also named as NEURL1, NEURL1A, RNF67.Neurl1 is a RING-dependent E3 ubiquitin ligase that can regulate Notch signaling in mammals and it expression promotes lysosomal degradation of Jagged1 in cells and blocks signaling from Jagged1-expressing cells to neighboring Notch-expressing cells. Neurl-dependent turnover of Jagged1 and inhibition of Notch signaling depends upon N-terminal myristoylation and plasma membrane localization of Neurl1(PMID:18077452).This antibody is specific to NEURL.

Protocols

Product Specific Protocols
WB protocol for NEURL antibody 18898-1-APDownload protocol
IHC protocol for NEURL antibody 18898-1-APDownload protocol
IF protocol for NEURL antibody 18898-1-APDownload protocol
IP protocol for NEURL antibody 18898-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Clin Invest

CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice.

Authors - Kun Zhang
  • KD Validated
View Article