Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells, mouse pancreas tissue, rat pancreas tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | rat brain tissue, human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 6 publications below |
| IF | See 2 publications below |
Product Information
12081-1-AP targets NEUROD1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NEUROD1 fusion protein Ag2713 相同性解析による交差性が予測される生物種 |
| Full Name | neurogenic differentiation 1 |
| Calculated molecular weight | 356 aa, 40 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC009046 |
| Gene Symbol | NEUROD1 |
| Gene ID (NCBI) | 4760 |
| RRID | AB_2877823 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13562 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NEUROD1 antibody 12081-1-AP | Download protocol |
| IHC protocol for NEUROD1 antibody 12081-1-AP | Download protocol |
| IF protocol for NEUROD1 antibody 12081-1-AP | Download protocol |
| IP protocol for NEUROD1 antibody 12081-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Prototypical oncogene family Myc defines unappreciated distinct lineage states of small cell lung cancer. | ||
Sci Adv Neurons burdened by DNA double-strand breaks incite microglia activation through antiviral-like signaling in neurodegeneration | ||
Biomaterials Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells. | ||
Proc Natl Acad Sci U S A CHD8 dosage regulates transcription in pluripotency and early murine neural differentiation. | ||