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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NEUROD2 Monoclonal antibody (68284-1-Ig)

    WB analysis using 68284-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68284-1-Ig (NEUROD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of mouse cerebellum tissue using NEUROD2 Monoclonal antibody (68284-1-Ig)

    WB analysis of mouse cerebellum using 68284-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68284-1-Ig (NEUROD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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NEUROD2 Monoclonal antibody

NEUROD2 Monoclonal Antibody for WB, ELISA
Cat No. 68284-1-Ig
Clone No.2C7B3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

WB, ELISA

Synonyms

bHLHa1, NDRF, NeuroD related factor, NEUROD2, neurogenic differentiation 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig brain tissue, mouse cerebellum tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rabbit cerebellum tissue, rat cerebellum tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68284-1-Ig targets NEUROD2 in WB, ELISA applications and shows reactivity with Human, mouse, pig samples.

Tested Reactivity Human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEUROD2 fusion protein Ag26068 相同性解析による交差性が予測される生物種
Full Name neurogenic differentiation 2
Calculated molecular weight 41 kDa
Observed molecular weight41-50 kDa
GenBank accession numberBC022481
Gene Symbol NEUROD2
Gene ID (NCBI) 4761
RRIDAB_2935365
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15784
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEUROD2 (Neurogenic differentiation factor 2), is transcriptional regulator that regulates early neuronal differentiation during development (PMID: 7754368). NeuroD2 is expressed in the postnatal cortex and mediate the activity-dependent refinement of thalamocortical axon terminals (PMID: 17453014). NeuroD2 is mainly involved in the development of the cerebellar and hippocampal granular neurons, neurons in the basolateral nucleus of amygdala and the hypothalamic-pituitary axis. Mutantions in NEUROD2 can cause neurodevelopmental disorders including intellectual disability and autism spectrum disorders (PMID: 34188164).

Protocols

Product Specific Protocols
WB protocol for NEUROD2 antibody 68284-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols