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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NEUROG1 Monoclonal antibody

NEUROG1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E1H6

Cat no : 66642-1-Ig

Synonyms

AKA, bHLHa6, Math4C, NEUROD3, NEUROG1, neurogenin 1, NGN, NGN 1, ngn1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of HEK-293 using 66642-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of Y79 using 66642-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of Neuro-2a using 66642-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of HeLa using 66642-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NEUROG1 Monoclonal antibody (66642-1-Ig)

    WB analysis of mouse brain using 66642-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66642-1-Ig (NEUROG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, Y79 cells, Neuro-2a cells, HeLa cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66642-1-Ig targets NEUROG1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NEUROG1 fusion protein Ag26438 相同性解析による交差性が予測される生物種
Full Name neurogenin 1
Calculated molecular weight 237 aa, 26 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC008687
Gene symbol NEUROG1
Gene ID (NCBI) 4762
RRIDAB_2882001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NEUROG1, also named BHLHA6, NEUROD3, NGN, and NGN1, is a 237 amino acid protein, which is expressed in embryonic nervous system. NEUROG1 acts as a transcriptional regulator that is involved in the initiation of neuronal differentiation. It activates transcription by binding to the E box (5'-CANNTG-3'). NEUROG1 can associate with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis.

Protocols

Product Specific Protocols
WB protocol for NEUROG1 antibody 66642-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols