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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NF-κB p65 Monoclonal antibody (66535-1-Ig)

    WB analysis of HEK-293 using 66535-1-Ig (same clone as 66535-1-PBS)

    WB result of p65; RELA antibody (66535-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p65; RELA transfected HEK-293 cells. This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NF-κB p65 Monoclonal antibody (66535-1-Ig)

    WB analysis using 66535-1-Ig (same clone as 66535-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66535-1-Ig (NF-κB p65 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF-κB p65 Monoclonal antibody (66535-1-Ig)

    IHC staining of human breast cancer using 66535-1-Ig (same clone as 66535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66535-1-Ig (p65; RELA antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF-κB p65 Monoclonal antibody (66535-1-Ig)

    IHC staining of human breast cancer using 66535-1-Ig (same clone as 66535-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66535-1-Ig (p65; RELA antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NF-κB p65 Monoclonal antibody (66535-1-Ig)

    IF Staining of HepG2 using 66535-1-Ig (same clone as 66535-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NF-κB p65 antibody (66535-1-Ig, Clone: 1B12D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66535-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NF-κB p65 Monoclonal antibody, PBS Only

NF-κB p65 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66535-1-PBS
Clone No.1B12D11

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

NF-kB, NFKB3, NF-κB, NF-κB p65, NF-κB3, p65, RELA, Transcription factor p65

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66535-1-PBS targets NF-κB p65 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NF-κB p65 fusion protein Ag1199 相同性解析による交差性が予測される生物種
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011603
Gene Symbol NF-κB p65
Gene ID (NCBI) 5970
RRIDAB_2881898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04206
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-Kb family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-Kb. The p65 subunit is a major component of NF-Kb complexes and is responsible for trans-activation. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. It associates with chromatin at the NF-kappa-B promoter region via association with DDX1.