NF-H/NF200 Polyclonal antibody, PBS Only
NF-H/NF200 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, Indirect ELISA
Conjugate
Unconjugated
Cat no : 18934-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
18934-1-PBS targets NF-H/NF200 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NF-H/NF200 fusion protein Ag13517 相同性解析による交差性が予測される生物種 |
Full Name | neurofilament, heavy polypeptide |
Calculated molecular weight | 112 kDa |
Observed molecular weight | 200 kDa, 140-160 kDa |
GenBank accession number | BC014185 |
Gene symbol | NF-H |
Gene ID (NCBI) | 4744 |
RRID | AB_10640801 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.