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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

    Various tissues were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    WB analysis of mouse brain tissue using 18934-1-AP (same clone as 18934-1-PBS)

    WB result of 18934-1-AP. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    WB analysis using 18934-1-AP (same clone as 18934-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    IHC staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18934-1-AP (NF-H/NF200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    IHC staining of human gliomas using 18934-1-AP (same clone as 18934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    IHC staining of human gliomas using 18934-1-AP (same clone as 18934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    IF Staining of rat brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using NF-H/NF200 Polyclonal antibody (18934-1-AP)

    IF Staining of mouse brain using 18934-1-AP (same clone as 18934-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NF-H/NF200 antibody (18934-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18934-1-PBS in a different storage buffer formulation.

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NF-H/NF200 Polyclonal antibody, PBS Only

NF-H/NF200 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 18934-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, neurofilament heavy chain

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18934-1-PBS targets NF-H/NF200 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-H/NF200 fusion protein Ag13517 相同性解析による交差性が予測される生物種
Full Name neurofilament, heavy polypeptide
Calculated molecular weight 112 kDa
Observed molecular weight 200 kDa, 140-160 kDa
GenBank accession numberBC014185
Gene Symbol NF-H
Gene ID (NCBI) 4744
RRIDAB_10640801
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12036
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.