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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NF-H/NF200 Monoclonal antibody, PBS Only

NF-H/NF200 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1A3C7

Cat no : 60331-1-PBS

Synonyms

NEFH, Neurofilament heavy polypeptide, NF200, NF-H, neurofilament heavy chain

Validation Data Gallery

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  • Western Blot (WB) analysis of pig cerebellum tissue using NF-H/NF200 Monoclonal antibody (60331-1-Ig)

    WB analysis of pig cerebellum using 60331-1-Ig (same clone as 60331-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using NF-H/NF200 Monoclonal antibody (60331-1-Ig)

    WB analysis of rat brain using 60331-1-Ig (same clone as 60331-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using NF-H/NF200 Monoclonal antibody (60331-1-Ig)

    WB analysis of mouse brain using 60331-1-Ig (same clone as 60331-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using NF-H/NF200 Monoclonal antibody (60331-1-Ig)

    IHC staining of rat cerebellum using 60331-1-Ig (same clone as 60331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 60331-1-Ig (NF-H antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using NF-H/NF200 Monoclonal antibody (60331-1-Ig)

    IF Staining of rat brain using 60331-1-Ig (same clone as 60331-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-H/NF200 antibody (60331-1-Ig, Clone: 1A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using NF-H/NF200 Monoclonal antibody (60331-1-Ig)

    FC experiment of SH-SY5Y using 60331-1-Ig (same clone as 60331-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NF-H/NF200 (60331-1-Ig, Clone:1A3C7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60331-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60331-1-PBS targets NF-H/NF200 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NF-H/NF200 fusion protein Ag13517 相同性解析による交差性が予測される生物種
Full Name neurofilament, heavy polypeptide
Calculated molecular weight 112 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC014185
Gene symbol NF-H
Gene ID (NCBI) 4744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.