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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NF2 Polyclonal antibody

NF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10659-1-AP

Synonyms

Neurofibromin-2, Neurofibromin 2, Moesin-ezrin-radixin-like protein, Merlin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NF2 Polyclonal antibody (10659-1-AP)

    WB analysis using 10659-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using NF2 Polyclonal antibody (10659-1-AP)

    WB analysis of mouse kidney using 10659-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NF2 Polyclonal antibody (10659-1-AP)

    WB analysis of Jurkat using 10659-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using NF2 Polyclonal antibody (10659-1-AP)

    WB analysis of NIH/3T3 using 10659-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using NF2 Polyclonal antibody (10659-1-AP)

    WB analysis of human kidney using 10659-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NF2 Polyclonal antibody (10659-1-AP)

    IHC staining of human stomach using 10659-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10659-1-AP (NF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inJurkat cells, human kidney tissue, mouse kidney tissue, NIH/3T3 cells, rat kidney tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10659-1-AP targets NF2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF2 fusion protein Ag1013 相同性解析による交差性が予測される生物種
Full Name neurofibromin 2 (merlin)
Calculated molecular weight 70 kDa
Observed molecular weight 64-66 kDa
GenBank accession numberBC007279
Gene symbol NF2
Gene ID (NCBI) 4771
RRIDAB_2149815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

Protocols

Product Specific Protocols
WB protocol for NF2 antibody 10659-1-APDownload protocol
IHC protocol for NF2 antibody 10659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

The histone lysine acetyltransferase KAT2B inhibits cholangiocarcinoma growth: evidence for interaction with SP1 to regulate NF2-YAP signaling

Authors - Wenbo Ma
View Article