Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HeLa using 21686-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 21686-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 21686-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 21686-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21686-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 21686-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 21686-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 21686-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21686-1-AP (NF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21686-1-AP
IP result of anti-NF2 (IP:21686-1-AP, 4ug; Detection:21686-1-AP 1:5000) with HeLa cells lysate 1560 ug.
IHC staining of mouse brain using 21686-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human meningioma using 21686-1-AP
Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human meningioma using 21686-1-AP
Immunohistochemical analysis of paraffin-embedded human meningioma tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse brain using 21686-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 21686-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse brain using 21686-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21686-1-AP (NF2 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 21686-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NF2 antibody (21686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 21686-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21686-1-AP (NF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

Neurofibromin 2 Polyclonal antibody

Neurofibromin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21686-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NF2, Neurofibromin-2, Moesin-ezrin-radixin-like protein, Merlin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, MDCK cells, mouse brain tissue, rat brain tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, human meningioma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:750-1:3000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

21686-1-AP targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity	human, mouse, rat, canine
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Neurofibromin 2 fusion protein Ag16265 相同性解析による交差性が予測される生物種
Full Name	neurofibromin 2 (merlin)
Calculated molecular weight	595 aa, 70 kDa
Observed molecular weight	66-70 kDa
GenBank accession number	BC020257
Gene Symbol	NF2
Gene ID (NCBI)	4771
RRID	AB_10792410
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35240
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

Protocols

Product Specific Protocols
WB protocol for Neurofibromin 2 antibody 21686-1-AP	Download protocol
IHC protocol for Neurofibromin 2 antibody 21686-1-AP	Download protocol
IF protocol for Neurofibromin 2 antibody 21686-1-AP	Download protocol
IP protocol for Neurofibromin 2 antibody 21686-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Thyroid MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer. Authors - Chunxiao Yu View Article
human	WB	J Cell Mol Med PMID: 32281270 Authors - Jie Liu View Article
human	IF	Onco Targets Ther PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin. Authors - Lai Wei KD Validated View Article
human	WB	Cancer Manag Res MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma. Authors - Hua-Chun Rao View Article
human	WB	Biochem Biophys Res Commun A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level. Authors - Yuen Gao View Article
rat	WB	J Cell Physiol Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan. Authors - Yan-Jing Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 21686-1-AP

WB analysis using 21686-1-AP

WB analysis of NIH/3T3 using 21686-1-AP

WB analysis of HepG2 using 21686-1-AP

WB analysis of MCF-7 using 21686-1-AP

WB analysis of HEK-293 using 21686-1-AP

WB analysis of Jurkat using 21686-1-AP

WB analysis of SH-SY5Y using 21686-1-AP

IP experiment of HeLa using 21686-1-AP

IHC staining of mouse brain using 21686-1-AP

IHC staining of human meningioma using 21686-1-AP

IHC staining of human meningioma using 21686-1-AP

IHC staining of mouse brain using 21686-1-AP

IHC staining of mouse brain using 21686-1-AP

IHC staining of mouse brain using 21686-1-AP

IF Staining of HeLa using 21686-1-AP

IF Staining of HeLa using 21686-1-AP

Neurofibromin 2 Polyclonal antibody

Neurofibromin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer.

PMID: 32281270

PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.

MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma.

A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level.

Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.