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  • KD/KO Validated

Neurofibromin 2 Polyclonal antibody

Neurofibromin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21686-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, ELISA

NF2, Neurofibromin-2, Moesin-ezrin-radixin-like protein, Merlin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, MDCK cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human meningioma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21686-1-AP targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Neurofibromin 2 fusion protein Ag16265 相同性解析による交差性が予測される生物種
Full Name neurofibromin 2 (merlin)
Calculated molecular weight 595 aa, 70 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC020257
Gene Symbol NF2
Gene ID (NCBI) 4771
RRIDAB_10792410
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

Protocols

Product Specific Protocols
WB protocol for Neurofibromin 2 antibody 21686-1-APDownload protocol
IHC protocol for Neurofibromin 2 antibody 21686-1-APDownload protocol
IF protocol for Neurofibromin 2 antibody 21686-1-APDownload protocol
IP protocol for Neurofibromin 2 antibody 21686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Thyroid

MicroRNA-146b-3p promotes cell metastasis by directly targeting NF2 in human papillary thyroid cancer.

Authors - Chunxiao Yu
humanWB

J Cell Mol Med

PMID: 32281270

Authors - Jie Liu
humanIF

Onco Targets Ther

PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.

Authors - Lai Wei
  • KD Validated
humanWB

Cancer Manag Res

MiR-25-3p Serves as an Oncogenic MicroRNA by Downregulating the Expression of Merlin in Osteosarcoma.

Authors - Hua-Chun Rao
humanWB

Biochem Biophys Res Commun

A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level.

Authors - Yuen Gao
ratWB

J Cell Physiol

Enhancement of tenogenic differentiation of rat tendon-derived stem cells by biglycan.

Authors - Yan-Jing Zhang
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