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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NF45 Polyclonal antibody

NF45 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24572-1-AP

Synonyms

ILF2, PRO3063, Nuclear factor of activated T-cells 45 kDa, Interleukin enhancer-binding factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using NF45 Polyclonal antibody (24572-1-AP)

    WB analysis of K-562 using 24572-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP (NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NF45 Polyclonal antibody (24572-1-AP)

    WB analysis of HepG2 using 24572-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24572-1-AP (NF45 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using NF45 Polyclonal antibody (24572-1-AP)

    IHC staining of human colon using 24572-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24572-1-AP (NF45 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF45 Polyclonal antibody (24572-1-AP)

    IHC staining of human breast cancer using 24572-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:200.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF45 Polyclonal antibody (24572-1-AP)

    IHC staining of human breast cancer using 24572-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:400.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF45 Polyclonal antibody (24572-1-AP)

    IHC staining of human breast cancer using 24572-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:400.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF45 Polyclonal antibody (24572-1-AP)

    IHC staining of human breast cancer using 24572-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF45 Polyclonal antibody (24572-1-AP)

    IHC staining of human breast cancer using 24572-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24572-1-AP (NF45 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NF45 Polyclonal antibody (24572-1-AP)

    IF Staining of HepG2 using 24572-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24572-1-AP (NF45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, HepG2 cells
Positive IHC detected inhuman colon tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24572-1-AP targets NF45 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF45 fusion protein Ag21480 相同性解析による交差性が予測される生物種
Full Name interleukin enhancer binding factor 2, 45kDa
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC000382
Gene symbol ILF2
Gene ID (NCBI) 3608
RRIDAB_2879615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-2 enhancer binding factor 2 (ILF2), also named as nuclear factor 45 (NF45), plays important roles in regulating interleukin-2 expression in mammals. It forms a heterodimeric complex with ILF3, which may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA [PMID:11739746,17129403].

Protocols

Product Specific Protocols
WB protocol for NF45 antibody 24572-1-APDownload protocol
IHC protocol for NF45 antibody 24572-1-APDownload protocol
IF protocol for NF45 antibody 24572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols