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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFAT5 Polyclonal antibody (21713-1-AP)

    WB analysis using 21713-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21713-1-AP (NFAT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of mouse skeletal muscle using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of mouse skeletal muscle using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of human urothelial carcinoma using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of human thyroid cancer using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of human thyroid cancer using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of rat skeletal muscle using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of mouse brain using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of mouse brain using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of rat brain using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using NFAT5 Polyclonal antibody (21713-1-AP)

    IHC staining of rat brain using 21713-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NFAT5 Polyclonal antibody (21713-1-AP)

    IF Staining of HepG2 using 21713-1-AP

    Immunofluorescent analysis of HepG2 cells using 21713-1-AP (NFAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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NFAT5 Polyclonal antibody

NFAT5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21713-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nuclear factor of activated T-cells 5, NFATZ, NFATL1, NF-AT5, NF AT5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, U2OS cells, COS-7 cells
Positive IHC detected inmouse skeletal muscle tissue, human urothelial carcinoma tissue, human thyroid cancer tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21713-1-AP targets NFAT5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFAT5 fusion protein Ag16341 相同性解析による交差性が予測される生物種
Full Name nuclear factor of activated T-cells 5, tonicity-responsive
Calculated molecular weight 1531 aa, 166 kDa
Observed molecular weight200 kDa
GenBank accession numberBC146765
Gene Symbol NFAT5
Gene ID (NCBI) 10725
RRIDAB_3085667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).

Protocols

Product Specific Protocols
WB protocol for NFAT5 antibody 21713-1-APDownload protocol
IHC protocol for NFAT5 antibody 21713-1-APDownload protocol
IF protocol for NFAT5 antibody 21713-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols