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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFATC1 Polyclonal antibody

NFATC1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30114-1-AP

Synonyms

NF ATC, NF ATc1, NFAT2, NFATc, NFATC1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFATC1 Polyclonal antibody (30114-1-AP)

    WB analysis using 30114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30114-1-AP (NFATC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using NFATC1 Polyclonal antibody (30114-1-AP)

    IF Staining of U-251 using 30114-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NFATC1 antibody (30114-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30114-1-AP targets NFATC1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFATC1 fusion protein Ag32365 相同性解析による交差性が予測される生物種
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
Calculated molecular weight716aa,78 kDa; 943aa,101 kDa
Observed molecular weight82-140 kDa
GenBank accession numberBC104753
Gene symbol NFATC1
Gene ID (NCBI) 4772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor of activated T-cells cytoplasmic (NFATC) is a family of transcription factors originally identified in T-cells. The family has five members (NFATC1 through NFATC5), which play roles both within and outside the immune system. NFATC1 is widely expressed and resides in endocardial cushion endothelial cells, skeletal muscle fibers, T lymphocytes, osteoblasts, osteoclasts, etc. The autoregulation of NFATC1 is a crucial step for cell fate determination. It is a master regulator of RANKL-induced osteoclast differentiation and plays a pivotal role in osteoclast fusion and osteoclast activation via up-regulation of various genes responsible for osteoclast adhesion, migration, acidification, degradation of inorganic and organic bone matrix (PMID: 20035895, PMID: 19754901). NFAT proteins is alternatively modified to generate splice variants. NFATC1 can be detected isoforms of 82-140 kDa (PMID: 18097033).

Protocols

Product Specific Protocols
WB protocol for NFATC1 antibody 30114-1-APDownload protocol
IF protocol for NFATC1 antibody 30114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols