• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

NFATC1 Monoclonal antibody, PBS Only

NFATC1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1B10

Cat no : 66963-1-PBS

Synonyms

Nuclear factor of activated T cells, cytoplasmic 1, NF-ATc1, NF-ATc, NFATc, NFAT2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NFATC1 Monoclonal antibody (66963-1-Ig)

    WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NFATC1 Monoclonal antibody (66963-1-Ig)

    WB analysis using 66963-1-Ig (same clone as 66963-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66963-1-Ig (NFATC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using NFATC1 Monoclonal antibody (66963-1-Ig)

    IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using NFATC1 Monoclonal antibody (66963-1-Ig)

    IHC staining of human urothelial carcinoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NFATC1 Monoclonal antibody (66963-1-Ig)

    IHC staining of human lymphoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NFATC1 Monoclonal antibody (66963-1-Ig)

    IHC staining of human lymphoma using 66963-1-Ig (same clone as 66963-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66963-1-Ig (NFATC1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66963-1-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66963-1-PBS targets NFATC1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFATC1 fusion protein Ag13014 相同性解析による交差性が予測される生物種
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1
Calculated molecular weight716aa,78 kDa; 943aa,101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC104753
Gene symbol NFATC1
Gene ID (NCBI) 4772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NFATc1(nuclear factor-activated T cells c1) is one of the transcription factors family consisting 5 members which were considered as a regulator of T-cell activation. In immune cell, NFATc1 is located in the cytoplasm in a hyperphosphorylated state and translocates into the nucleus upon cell stimulation via dephosphorylation(PMID:28327458). One paper observed that ectopic expression and nuclear accumulation of NFATc1 in more than 60% of human pancreatic cancers (PMID:25623042). Several alternative splicing transcript variants encoding different isoforms have been identified for this gene. And different isoforms of this protein may regulate different cytokine gene expression. NFATc1 is a main transcriptional activator for cytokine genes in activated lymphoid cells. Also some studies demonstrated that NFATc1 plays the role of a master transcription regulator of osteoclast differentiation (PMID:25489571).