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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFATC2 Monoclonal antibody, PBS Only

NFATC2 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3B2

Cat no : 66917-1-PBS

Synonyms

NFAT1, NFATP, NFAT pre-existing subunit, NFAT pre existing subunit, NF ATp

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFATC2 Monoclonal antibody (66917-1-Ig)

    WB analysis using 66917-1-Ig (same clone as 66917-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66917-1-Ig (NFATC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NFATC2 Monoclonal antibody (66917-1-Ig)

    IHC staining of human breast cancer using 66917-1-Ig (same clone as 66917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NFATC2 Monoclonal antibody (66917-1-Ig)

    IHC staining of mouse testis using 66917-1-Ig (same clone as 66917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NFATC2 Monoclonal antibody (66917-1-Ig)

    IHC staining of mouse testis using 66917-1-Ig (same clone as 66917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NFATC2 Monoclonal antibody (66917-1-Ig)

    IHC staining of human lymphoma using 66917-1-Ig (same clone as 66917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using NFATC2 Monoclonal antibody (66917-1-Ig)

    IHC staining of human lymphoma using 66917-1-Ig (same clone as 66917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NFATC2 Monoclonal antibody (66917-1-Ig)

    IHC staining of human breast cancer using 66917-1-Ig (same clone as 66917-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66917-1-Ig (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using NFATC2 Monoclonal antibody (66917-1-Ig)

    FC experiment of Jurkat using 66917-1-Ig (same clone as 66917-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug Anti-Human NFATC2 (66917-1-Ig, Clone:1F3B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66917-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66917-1-PBS targets NFATC2 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFATC2 fusion protein Ag17990 相同性解析による交差性が予測される生物種
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
Calculated molecular weight 925 aa, 100 kDa
Observed molecular weight 135-140 kDa
GenBank accession numberBC136418
Gene symbol NFATC2
Gene ID (NCBI) 4773
RRIDAB_2882244
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.