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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFATC3 Monoclonal antibody (66924-1-Ig)

    WB analysis using 66924-1-Ig (same clone as 66924-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66924-1-Ig (NFATC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve of MP50022-1

    Sandwich ELISA standard curve ofMP50022-1, NFATC3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66924-2-PBS. Detection antibody: HRP-conjugated 66924-1-PBS. Standard: Ag27306. Range: 0.625-20 ng/mL.

  • Immunohistochemistry (IHC) staining of human thymus tissue using NFATC3 Monoclonal antibody (66924-1-Ig)

    IHC staining of human thymus using 66924-1-Ig (same clone as 66924-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thymus tissue using NFATC3 Monoclonal antibody (66924-1-Ig)

    IHC staining of human thymus using 66924-1-Ig (same clone as 66924-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 66924-1-Ig (NFATC3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66924-1-PBS in a different storage buffer formulation.

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NFATC3 Monoclonal antibody, PBS Only (Detector)

NFATC3 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA
Cat No. 66924-1-PBS
Clone No.1C1H8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

NF AT4, NF ATc3, NFAT4, NFATC3, NFATX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66924-1-PBS targets NFATC3 as part of a matched antibody pair:

MP50022-1: 66924-2-PBS capture and 66924-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Affinity
KD=1.16 x 10-10M
KOff=5.67 x 10-6M
KOn=4.90 x 104M
Immunogen NFATC3 fusion protein Ag27306 相同性解析による交差性が予測される生物種
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
Calculated molecular weight 116 kDa
Observed molecular weight 160 kDa
GenBank accession numberBC001050
Gene Symbol NFATC3
Gene ID (NCBI) 4775
RRIDAB_2882251
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ12968
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NFAT (nuclear factors of activated T cells) proteins are a family of transcription factors originally identified as mediators of activation of cytokine genes in response to antigenic stimulation of T cells. NFAT proteins also play varied roles in cells outside of the immune system [PMID:11877454]. NFATc3 has specifically been implicated in vasculature development, regulation of smooth muscle contractile phenotype, and modulation of vascular smooth muscle contractility [PMID:11439183,17148444]. The calculated molecular weight of NFATC3 is 115 kDa, but the post-modified protein is about 130-170 kDa (PMID: 15728531, PMID: 15857835)