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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFATC4 Polyclonal antibody

NFATC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26311-1-AP

Synonyms

NF AT3, NF ATc4, NFAT3, NFATC4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFATC4 Polyclonal antibody (26311-1-AP)

    WB analysis using 26311-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26311-1-AP (NFATC4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NFATC4 Polyclonal antibody (26311-1-AP)

    WB analysis using 26311-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26311-1-AP (NFATC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NFATC4 Polyclonal antibody (26311-1-AP)

    IHC staining of human lung cancer using 26311-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26311-1-AP (NFATC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NFATC4 Polyclonal antibody (26311-1-AP)

    IHC staining of human lung cancer using 26311-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26311-1-AP (NFATC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26311-1-AP targets NFATC4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFATC4 fusion protein Ag24503 相同性解析による交差性が予測される生物種
Full Name nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4
Calculated molecular weight 95 kDa
Observed molecular weight 90-105 kDa
GenBank accession numberBC053855
Gene symbol NFATC4
Gene ID (NCBI) 4776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFATC4, also named NFAT3, is a member from the Nuclear Factor of Activated T cells (NFATs) transcription factor family. NFATc4 contains a conserved Rel homology domain (RHD) which includes the DNA-binding motif, and two transactivation domains (TADs) which locate at the N-terminus and C-terminus respectively. NFATC4 is involved in several physiological processes, including the development and function of the immune, cardiovascular, musculoskeletal, and nervous systems. It is also reported that NFATC4 could take part in cell growth, cell migration, tumor metastasis, and patient survival recently.

Protocols

Product Specific Protocols
WB protocol for NFATC4 antibody 26311-1-APDownload protocol
IHC protocol for NFATC4 antibody 26311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols