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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using NFE2 Monoclonal antibody (66436-1-Ig)

    WB analysis of HL-60 using 66436-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NFE2 Monoclonal antibody (66436-1-Ig)

    WB analysis of K-562 using 66436-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66436-1-Ig (NFE2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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NFE2 Monoclonal antibody

NFE2 Monoclonal Antibody for WB, ELISA
Cat No. 66436-1-Ig
Clone No.1D7C8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

p45, Nuclear factor, erythroid-derived 2 45 kDa subunit, NF E2, Leucine zipper protein NF-E2, Leucine zipper protein NF E2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66436-1-Ig targets NFE2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFE2 fusion protein Ag1570 相同性解析による交差性が予測される生物種
Full Name nuclear factor (erythroid-derived 2), 45kDa
Calculated molecular weight 41 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC005044
Gene Symbol NFE2
Gene ID (NCBI) 4778
RRIDAB_2881806
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16621
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The transcription factor p45 nuclear factor-erythroid-derived 2(NFE2) is one component of the NF-E2 complex that essential for regulating reythroid and megakaryocytic maturation and differentiation. It also plays crucial roles in erythroid and megakaryocytic lineages. NFE2 recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters, and acts as a activator. And it may has a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Protocols

Product Specific Protocols
WB protocol for NFE2 antibody 66436-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols