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KD/KO Validated

NFE2L1 Polyclonal antibody

Name: NFE2L1 Polyclonal antibody
Brand: Proteintech
SKU: 12936-1-AP

NFE2L1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, chIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12936-1-AP

Synonyms

HBZ17, LCR F1, Locus control region factor 1, NF E2 related factor 1, NFE2 related factor 1, NFE2L1, NRF1, TCF 11, TCF11, Transcription factor 11, Transcription factor HBZ17, Transcription factor LCR F1

Validation Data Gallery

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AllWBIPIHC

WB analysis of mouse skeletal muscle using 12936-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12936-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 12936-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12936-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12936-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12936-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12936-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12936-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12936-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12936-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12936-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Fetal mouse bone using 12936-1-AP
Fetal mouse bone were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.
WB analysis of Fetal mouse bone using 12936-1-AP
Fetal mouse bone were subjected to SDS PAGE followed by western blot with 12936-1-AP (NFE2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 12936-1-AP
IP result of anti-NFE2L1 (IP:12936-1-AP, 4ug; Detection:12936-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.
IHC staining of human breast cancer using 12936-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12936-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12936-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12936-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12936-1-AP (NFE2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 12936-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse skeletal muscle tissue, Fetal mouse bone tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human breast cancer tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 13 publications below
IF	See 1 publications below
IP	See 1 publications below
ChIP	See 1 publications below

Product Information

12936-1-AP targets NFE2L1 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NFE2L1 fusion protein Ag3916 相同性解析による交差性が予測される生物種
Full Name	nuclear factor (erythroid-derived 2)-like 1
Calculated molecular weight	772 aa, 85 kDa
Observed molecular weight	65 kDa
GenBank accession number	BC010623
Gene symbol	NFE2L1
Gene ID (NCBI)	4779
RRID	AB_2267298
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NFE2L1, also named as HBZ17, NRF1, TCF11 and LCR-F1, belongs to the bZIP family and CNC subfamily. It activates erythroid-specific, globin gene expression. NFE2L1 is involved in mediating ascorbic acid-induced osterix expression and osteoblast differentiation via binding to the antioxidant response element of the osterix promoter. NFE2L1, produced by osteoblasts, is involved in regulating osterix expression, osteoblast differentiation, and bone formation. (PMID:19887580). This antibody is a rabbit polyclonal antibody raised against a region of human NFE2L1.

Protocols

Product Specific Protocols
WB protocol for NFE2L1 antibody 12936-1-AP	Download protocol
IHC protocol for NFE2L1 antibody 12936-1-AP	Download protocol
IP protocol for NFE2L1 antibody 12936-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma. Authors - Tian-Yi Jiang View Article
mouse	WB,IP	Sci Adv Lipid-anchored proteasomes control membrane protein homeostasis Authors - Ruizhu Zhang View Article
mouse	WB	Redox Biol Long isoforms of NRF1 negatively regulate adipogenesis via suppression of PPARγ expression. Authors - Peng Xue View Article
mouse	WB	Free Radic Biol Med Novel mechanistic insight on the neuroprotective effect of berberine: The role of PPARδ for antioxidant action. Authors - Jia-Wen Shou View Article
human	WB	Ecotoxicol Environ Saf Long-isoform NFE2L1 silencing inhibits acquisition of malignant phenotypes induced by arsenite in human bronchial epithelial cells. Authors - Yongfang Li KO Validated View Article
mouse	WB	Food Chem Toxicol Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice Authors - Simeng Bao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFE2L1 Polyclonal antibody

NFE2L1 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse skeletal muscle using 12936-1-AP

WB analysis of K-562 using 12936-1-AP

WB analysis of mouse skeletal muscle using 12936-1-AP

WB analysis of HepG2 using 12936-1-AP

WB analysis of Jurkat using 12936-1-AP

WB analysis of HeLa using 12936-1-AP

WB analysis of HepG2 using 12936-1-AP

WB analysis of K-562 using 12936-1-AP

WB analysis of Jurkat using 12936-1-AP

WB analysis of HeLa using 12936-1-AP

WB analysis of K-562 using 12936-1-AP

WB analysis of Fetal mouse bone using 12936-1-AP

WB analysis of Fetal mouse bone using 12936-1-AP

IP experiment of mouse skeletal muscle using 12936-1-AP

IHC staining of human breast cancer using 12936-1-AP

IHC staining of mouse brain using 12936-1-AP

IHC staining of mouse brain using 12936-1-AP

IHC staining of human breast cancer using 12936-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Lipid-anchored proteasomes control membrane protein homeostasis

Long isoforms of NRF1 negatively regulate adipogenesis via suppression of PPARγ expression.

Novel mechanistic insight on the neuroprotective effect of berberine: The role of PPARδ for antioxidant action.

Long-isoform NFE2L1 silencing inhibits acquisition of malignant phenotypes induced by arsenite in human bronchial epithelial cells.

Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice