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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NFE2L1 Recombinant antibody (83987-2-RR)

    IF Staining of HepG2 using 83987-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFE2L1 antibody (83987-2-RR, Clone: 241089D1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83987-2-RR

    Affinity and Kinetic Characterization of 83987-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83987-2-RR against Human NFE2L1 were performed. The affinity constant is 0.988 nM.

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NFE2L1 Recombinant antibody

NFE2L1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA
Cat No. 83987-2-RR
Clone No.241089D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

NF-E2-related factor 1, Locus control region-factor 1, LCR-F1, HBZ17, Endoplasmic reticulum membrane sensor NFE2L1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83987-2-RR targets NFE2L1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NFE2L1 fusion protein Ag0525 相同性解析による交差性が予測される生物種
Full Name nuclear factor (erythroid-derived 2)-like 1
Calculated molecular weight 772 aa, 85 kDa
GenBank accession numberBC010623
Gene Symbol NFE2L1
Gene ID (NCBI) 4779
RRIDAB_3671562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14494
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for NFE2L1 antibody 83987-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols