Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of A431 using 11750-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11750-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11750-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11750-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11750-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11750-1-AP (NFIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 11750-1-AP
IP result of anti-NFIA (IP:11750-1-AP, 4ug; Detection:11750-1-AP 1:500) with A431 cells lysate 2000ug.
IHC staining of mouse brain using 11750-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 11750-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11750-1-AP (NFIA antibody) at dilution of 1:25 (under 10x lens).
IHC staining of human gliomas using 11750-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11750-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11750-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11750-1-AP (NFIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NFIA Polyclonal antibody

NFIA Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11750-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, CoIP, ELISA

Synonyms

CTF, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, Jurkat cells, L02 cells, mouse liver tissue
Positive IP detected in	A431 cells
Positive IHC detected in	mouse brain tissue, human prostate cancer tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
CoIP	See 1 publications below

Product Information

11750-1-AP targets NFIA in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NFIA fusion protein Ag2346 相同性解析による交差性が予測される生物種
Full Name	nuclear factor I/A
Calculated molecular weight	498 aa, 55 kDa
Observed molecular weight	60-70 kDa
GenBank accession number	BC022264
Gene Symbol	NFIA
Gene ID (NCBI)	4774
RRID	AB_2153214
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12857
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. Catalog# 11750-1-AP is a rabbit polyclonal antibody raised against N-terminal of human original NFIA. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)

Protocols

Product Specific Protocols
WB protocol for NFIA antibody 11750-1-AP	Download protocol
IHC protocol for NFIA antibody 11750-1-AP	Download protocol
IP protocol for NFIA antibody 11750-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Cycle TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs. Authors - Daniel J Foster View Article
human	WB	Cancer Cell Int Macrophages-derived exosomal lncRNA LIFR-AS1 promotes osteosarcoma cell progression via miR-29a/NFIA axis. Authors - Hongliang Zhang View Article
mouse	IHC	FEBS Lett STAT3 transcriptionally regulates the expression of genes related to glycogen metabolism in developing motor neurons Authors - Hitoshi Gotoh View Article
human	WB,CoIP	Pathol Res Pract SOX9/NFIA promotes human ovarian cancer metastasis through the Wnt/β-catenin signaling pathway Authors - Rong Lu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 11750-1-AP

WB analysis of L02 using 11750-1-AP

WB analysis of mouse liver using 11750-1-AP

WB analysis of HeLa using 11750-1-AP

WB analysis of Jurkat using 11750-1-AP

IP experiment of A431 using 11750-1-AP

IHC staining of mouse brain using 11750-1-AP

IHC staining of human prostate cancer using 11750-1-AP

IHC staining of human gliomas using 11750-1-AP