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NFIA Monoclonal antibody

NFIA Monoclonal Antibody for WB, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig, rabbit and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3A6D7

Cat no : 66999-1-Ig

Synonyms

CTF, DKFZp434L0422, DKFZp686J23256, FLJ39164, KIAA1439, NF I/A, NF1 A, NFI A, NFI L, NFIA, Nuclear factor 1 A type, Nuclear factor 1/A, nuclear factor I/A, TGGCA binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFIA Monoclonal antibody (66999-1-Ig)

    WB analysis using 66999-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of MCF-7 cells using NFIA Monoclonal antibody (66999-1-Ig)

    WB analysis of MCF-7 using 66999-1-Ig

    WB result of NFIA antibody (66999-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NFIA transfected MCF-7 cells.

  • Western Blot (WB) analysis of HeLa cells using NFIA Monoclonal antibody (66999-1-Ig)

    WB analysis of HeLa using 66999-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NFIA Monoclonal antibody (66999-1-Ig)

    WB analysis using 66999-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using NFIA Monoclonal antibody (66999-1-Ig)

    WB analysis of mouse cerebellum using 66999-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66999-1-Ig (NFIA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NFIA Monoclonal antibody (66999-1-Ig)

    IP experiment of HeLa using 66999-1-Ig

    IP result of anti-NFIA (IP:66999-1-Ig, 5ug; Detection:66999-1-Ig 1:500) with HeLa cells lysate 1600 ug.

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Tested Applications

Positive WB detected inHeLa cells, mouse cerebellum tissue, fetal human brain tissue, Jurkat cells, pig brain tissue, rat brain tissue, mouse brain tissue, rabbit brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66999-1-Ig targets NFIA in WB, IP, ELISA applications and shows reactivity with Human, rat, mouse, pig, rabbit samples.

Tested Reactivity Human, rat, mouse, pig, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NFIA fusion protein Ag17873 相同性解析による交差性が予測される生物種
Full Name nuclear factor I/A
Calculated molecular weight 498 aa, 55 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC022264
Gene symbol NFIA
Gene ID (NCBI) 4774
RRIDAB_2882316
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The NFI (nuclear factor I) family consists of four members in vertebrates (NFI-A, NFI-B, NFI-C and NFI-X), and the four NFI genes are expressed in unique patterns during mouse embryogenesis and in the adult. Four isoforms of NFIA were found in human and they play various roles in DNA replication, DNA-dependent transcritpion via their DNA binding property. Multiple residues of NFIA can be phosphorylated resulting in mild shifts of its practical molecular weight. Recent finding also revealed its neuroprotective function in NMDA-induced neuronal damage. The calculated molecular weight of NFIA is 55 kDa, However the size of the proteins cross-linked to the adenoviral NF-I element ranged from 60 to 80 kDa . The larger size observed by us could be due to the oligo protein complex, which would increase the size by 15-20 kDa. (PMID: 11447215)

Protocols

Product Specific Protocols
WB protocol for NFIA antibody 66999-1-IgDownload protocol
IP protocol for NFIA antibody 66999-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Transl Med

Single-cell multiomics guided mechanistic understanding of Fontan-associated liver disease

Authors - Po Hu
View Article