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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFIC Monoclonal antibody (68318-1-Ig)

    WB analysis using 68318-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using NFIC Monoclonal antibody (68318-1-Ig)

    WB analysis using 68318-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68318-1-Ig (NFIC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using NFIC Monoclonal antibody (68318-1-Ig)

    FC experiment of HeLa using 68318-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFIC Monoclonal antibody (68318-1-Ig, Clone:1A11A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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NFIC Monoclonal antibody

NFIC Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68318-1-Ig
Clone No.1A11A5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

NF-I/C, NF1-C, NF I, CTF, CCAAT-box-binding transcription factor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHSC-T6 cells, Saos-2 cells, U2OS cellls, LNCaP cells, HeLa cells, NIH/3T3 cells, 4T1 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68318-1-Ig targets NFIC in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NFIC fusion protein Ag9472 相同性解析による交差性が予測される生物種
Full Name nuclear factor I/C (CCAAT-binding transcription factor)
Calculated molecular weight428aa,48 kDa; 508aa,57 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC012120
Gene Symbol NFIC
Gene ID (NCBI) 4782
RRIDAB_3085054
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08651
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFIC, also named as Nuclear factor 1 C-type, is a 508 amino acid protein, which contains 1 CTF/NF-I DNA-binding domain and belongs to the CTF/NF-I family. NFIC localizes in the nucleus and recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Protocols

Product Specific Protocols
WB protocol for NFIC antibody 68318-1-IgDownload protocol
FC protocol for NFIC antibody 68318-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols