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KD/KO Validated

NFIL3 Polyclonal antibody

Name: NFIL3 Polyclonal antibody
Brand: Proteintech
SKU: 11773-1-AP

NFIL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11773-1-AP

Synonyms

NFIL3A, Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11773-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11773-1-AP
IP result of anti-NFIL3 (IP:11773-1-AP, 4ug; Detection:11773-1-AP 1:1000) with HeLa cells lysate 1600ug.
IHC staining of mouse stomach using 11773-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 11773-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 11773-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11773-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11773-1-AP (NFIL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of NK92 using 11773-1-AP
1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (11773-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 11773-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, K562 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse stomach tissue, human skin cancer tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	NK92, NK92 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 13 publications below
IHC	See 5 publications below
IF	See 3 publications below
IP	See 1 publications below
ChIP	See 5 publications below

Product Information

11773-1-AP targets NFIL3 in WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NFIL3 fusion protein Ag2380 相同性解析による交差性が予測される生物種
Full Name	nuclear factor, interleukin 3 regulated
Calculated molecular weight	462 aa, 51 kDa
Observed molecular weight	55-60 kDa
GenBank accession number	BC008197
Gene symbol	NFIL3
Gene ID (NCBI)	4783
RRID	AB_2267287
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NFIL3, also named as E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227). The calculated molecular weight of NFIL3 is 51 kDa, but the modified NFIL3 is about 55-60 kDa.

Protocols

Product Specific Protocols
WB protocol for NFIL3 antibody 11773-1-AP	Download protocol
IHC protocol for NFIL3 antibody 11773-1-AP	Download protocol
IF protocol for NFIL3 antibody 11773-1-AP	Download protocol
IP protocol for NFIL3 antibody 11773-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Brain Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice. Authors - Gen Shiihashi View Article
mouse		Theranostics The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition. Authors - Fangjun Yu View Article
rat	WB	Neuropharmacology Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area. Authors - Cai Li View Article
mouse	WB	Biochem Pharmacol Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice Authors - Min Chen View Article
human	WB, ChIP	Front Med (Lausanne) NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation. Authors - Honghu Tang View Article
mouse	WB,chIP	Drug Metab Dispos The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver. Authors - Min Chen KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFIL3 Polyclonal antibody

NFIL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 11773-1-AP

IP experiment of HeLa using 11773-1-AP

IHC staining of mouse stomach using 11773-1-AP

IHC staining of rat stomach using 11773-1-AP

IHC staining of human skin cancer using 11773-1-AP

IF Staining of HepG2 using 11773-1-AP

FC experiment of NK92 using 11773-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.

The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.

Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area.

Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice

NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation.

The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.