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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFKB1,p105,p50 Polyclonal antibody, PBS Only

NFKB1,p105,p50 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14220-1-PBS

Synonyms

NFKB1, NF kappa B, KBF1, EBP-1, EBP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFKB1,p105,p50 Polyclonal antibody (14220-1-AP)

    WB analysis using 14220-1-AP (same clone as 14220-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14220-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NFKB1,p105,p50 Polyclonal antibody (14220-1-AP)

    WB analysis using 14220-1-AP (same clone as 14220-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14220-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using NFKB1,p105,p50 Polyclonal antibody (14220-1-AP)

    IP experiment of Jurkat using 14220-1-AP (same clone as 14220-1-PBS)

    IP result of anti-NFKB1,p105,p50 (IP:14220-1-AP, 4ug; Detection:14220-1-AP 1:3000) with Jurkat cells lysate 2280 ug. This data was developed using the same antibody clone with 14220-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NFKB1,p105,p50 Polyclonal antibody (14220-1-AP)

    IF Staining of HepG2 using 14220-1-AP (same clone as 14220-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14220-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NFKB1,p105,p50 Polyclonal antibody (14220-1-AP)

    IF Staining of HeLa using 14220-1-AP (same clone as 14220-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NFKB1 antibody (14220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14220-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using NFKB1,p105,p50 Polyclonal antibody (14220-1-AP)

    FC experiment of HepG2 using 14220-1-AP (same clone as 14220-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (14220-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 14220-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14220-1-PBS targets NFKB1,p105,p50 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFKB1,p105,p50 fusion protein Ag5458 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Calculated molecular weight 105 kDa
Observed molecular weight 50 kDa, 105 kDa
GenBank accession numberBC051765
Gene symbol NFKB1
Gene ID (NCBI) 4790
RRIDAB_2153393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.