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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFKB2,p100 Recombinant antibody, PBS Only

NFKB2,p100 Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241126C9

Cat no : 84022-5-PBS

Synonyms

NFKB2, LYT10, LYT 10, H2TF1, DNA-binding factor KBF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NFKB2,p100 Recombinant antibody (84022-5-RR)

    WB analysis using 84022-5-RR (same clone as 84022-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84022-5-RR (NFKB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84022-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NFKB2,p100 Recombinant antibody (84022-5-RR)

    FC experiment of HeLa using 84022-5-RR (same clone as 84022-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NFKB2,p100 Recombinant antibody (84022-5-RR, Clone:241126C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84022-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using NFKB2,p100 Recombinant antibody (84022-5-RR)

    FC experiment of A431 using 84022-5-RR (same clone as 84022-5-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NFKB2,p100 Recombinant antibody (84022-5-RR, Clone:241126C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84022-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84022-5-RR

    Affinity and Kinetic Characterization of 84022-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84022-5-RR against Human NFKB2,p100 were performed. The affinity constant is 0.109 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84022-5-PBS is NFKB2,p100 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NFKB2,p100 fusion protein Ag0662 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Calculated molecular weight 97 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC002844
Gene symbol NFKB2
Gene ID (NCBI) 4791
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.