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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IkB Beta Polyclonal antibody

IkB Beta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12660-1-AP

Synonyms

IκB beta, IκB β, NFKBIB, IkB-B, IKBB

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IkB Beta Polyclonal antibody (12660-1-AP)

    WB analysis using 12660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using IkB Beta Polyclonal antibody (12660-1-AP)

    WB analysis of MCF-7 using 12660-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12660-1-AP (IkB beta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human colon using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human colon using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human colon cancer using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human endometrial cancer using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human endometrial cancer using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 12660-1-AP (IkB beta antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human kidney using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using IkB Beta Polyclonal antibody (12660-1-AP)

    IHC staining of human kidney using 12660-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12660-1-AP (IkB beta antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IkB Beta Polyclonal antibody (12660-1-AP)

    IF Staining of HeLa using 12660-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IkB Beta antibody (12660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IHC detected inhuman colon tissue, human endometrial cancer tissue, human colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12660-1-AP targets IkB Beta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IkB Beta fusion protein Ag3340 相同性解析による交差性が予測される生物種
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Calculated molecular weight 356 aa, 38 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC015528
Gene symbol IkB Beta
Gene ID (NCBI) 4793
RRIDAB_2151611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IkB Beta antibody 12660-1-APDownload protocol
IHC protocol for IkB Beta antibody 12660-1-APDownload protocol
IF protocol for IkB Beta antibody 12660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Toxicol Appl Pharmacol

Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells.

Authors - Azubuike Peter Ebokaiwe
View Article
mouseWB

Gut Microbes

Gut microbiota regulates host melatonin production through epithelial cell MyD88

Authors - Bingnan Liu
View Article