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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFU1 Polyclonal antibody

NFU1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 25598-1-AP

Synonyms

CGI 33, HIRA interacting protein 5, HIRIP5, Nfu, NFU1, NifU, NIFUC

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using NFU1 Polyclonal antibody (25598-1-AP)

    WB analysis of mouse cerebellum using 25598-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NFU1 Polyclonal antibody (25598-1-AP)

    WB analysis of mouse brain using 25598-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse cerebellum tissue using NFU1 Polyclonal antibody (25598-1-AP)

    WB analysis of mouse cerebellum using 25598-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse testis tissue using NFU1 Polyclonal antibody (25598-1-AP)

    WB analysis of mouse testis using 25598-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse brain tissue using NFU1 Polyclonal antibody (25598-1-AP)

    WB analysis of mouse brain using 25598-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25598-1-AP (NFU1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NFU1 Polyclonal antibody (25598-1-AP)

    IP experiment of mouse brain using 25598-1-AP

    IP result of anti-NFU1 (IP:25598-1-AP, 4ug; Detection:25598-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NFU1 Polyclonal antibody (25598-1-AP)

    IF Staining of HepG2 using 25598-1-AP

    Immunofluorescent analysis of HepG2 cells using 25598-1-AP (NFU1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25598-1-AP is NFU1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFU1 fusion protein Ag22343 相同性解析による交差性が予測される生物種
Full Name NFU1 iron-sulfur cluster scaffold homolog (S. cerevisiae)
Calculated molecular weight 254 aa, 28 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC113692
Gene symbol NFU1
Gene ID (NCBI) 27247
RRIDAB_2880151
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFU1, also named as HIRA-interacting protein 5 or CGI-33, is a 254 amino acid protein, which belongs to the NifU family. NFU1 localizes in the Mitochondrion. NFU1 is widely expressed in adult tissues. NFU1, as an Iron-sulfur cluster scaffold protein, which can assemble clusters and deliver them to target proteins. It has an association with Multiple mitochondrial dysfunctions syndrome 1.

Protocols

Product Specific Protocols
WB protocol for NFU1 antibody 25598-1-APDownload protocol
IF protocol for NFU1 antibody 25598-1-APDownload protocol
IP protocol for NFU1 antibody 25598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
View Article