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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFX1 Recombinant antibody, PBS Only

NFX1 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230211D9

Cat no : 82924-1-PBS

Synonyms

Transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, NFX2, EC:2.3.2.-

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NFX1 Recombinant antibody (82924-1-RR)

    IF Staining of HeLa using 82924-1-RR (same clone as 82924-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFX1 antibody (82924-1-RR, Clone: 230211D9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82924-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using NFX1 Recombinant antibody (82924-1-RR)

    FC experiment of PC-3 using 82924-1-RR (same clone as 82924-1-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug NFX1 Recombinant antibody (82924-1-RR, Clone:230211D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82924-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82924-1-PBS targets NFX1 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NFX1 fusion protein Ag9448 相同性解析による交差性が予測される生物種
Full Name nuclear transcription factor, X-box binding 1
Calculated molecular weight 1120 aa, 124 kDa
GenBank accession numberBC012151
Gene symbol NFX1
Gene ID (NCBI) 4799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.