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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFX1 Recombinant antibody, PBS Only (Capture)

NFX1 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230211A10

Cat no : 82924-3-PBS

Synonyms

Transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, NFX2, EC:2.3.2.-

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00127-4

    Sandwich ELISA standard curve of MP00127-4

    Sandwich ELISA standard curve of MP00127-4, Human NFX1 Recombinant Matched Antibody Pair - PBS only. 82924-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9448. 82924-7-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

  • Sample test of MP00127-4

    Sample test of MP00127-4

    HEK-293T and Jurkat cell lysate were measured. The human NFX1 concentration of detected samples was determined to be 6,486.4 pg/mL in HEK-293T cell lysate (based on a 1.4 mg/mL extract load) and 5,377.9 pg/mL in Jurkat cell lysate (based on a 1.0 mg/mL extract load).

  • Cytometric bead array standard curve of MP00127-1

    Cytometric bead array standard curve of MP00127-1

    Cytometric bead array standard curve of MP00127-1, NFX1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82924-3-PBS. Detection antibody: 82924-4-PBS. Standard: Ag9448. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00127-2

    Cytometric bead array standard curve of MP00127-2

    Cytometric bead array standard curve of MP00127-2, NFX1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82924-3-PBS. Detection antibody: 82924-6-PBS. Standard: Ag9448. Range: 0.313-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82924-3-PBS targets NFX1 as part of a matched antibody pair:

MP00127-1: 82924-3-PBS capture and 82924-4-PBS detection (validated in Cytometric bead array)

MP00127-2: 82924-3-PBS capture and 82924-6-PBS detection (validated in Cytometric bead array)

MP00127-4: 82924-3-PBS capture and 82924-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NFX1 fusion protein Ag9448 相同性解析による交差性が予測される生物種
Full Name nuclear transcription factor, X-box binding 1
Calculated molecular weight 1120 aa, 124 kDa
GenBank accession numberBC012151
Gene symbol NFX1
Gene ID (NCBI) 4799
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.