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KD/KO Validated

NFYA Polyclonal antibody

Name: NFYA Polyclonal antibody
Brand: Proteintech
SKU: 12981-1-AP

NFYA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12981-1-AP

Synonyms

NF-YA, NF YA, HAP2, CBF B, CBF A

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of HEK-293 using 12981-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 12981-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12981-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12981-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12981-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 12981-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12981-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 12981-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 12981-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12981-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFYA antibody (12981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Cat No. 12981-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HT-1080 cells, Jurkat cells, K-562 cells
Positive IHC detected in	human colon cancer tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 3 publications below
IHC	See 1 publications below

Product Information

12981-1-AP targets NFYA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NFYA fusion protein Ag3604 相同性解析による交差性が予測される生物種
Full Name	nuclear transcription factor Y, alpha
Calculated molecular weight	318aa,34 kDa; 347aa,39 kDa
Observed molecular weight	40 kDa, 46 kDa
GenBank accession number	BC039244
Gene symbol	NFYA
Gene ID (NCBI)	4800
RRID	AB_10644128
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NFYA, also named as Nuclear transcription factor Y subunit alpha, is a 347 amino acid protein, which contains 1 NFYA/HAP2-type DNA-binding domain and belongs to the NFYA/HAP2 subunit family. NFYA exists the two 41- to 45-kDa isoforms of NF-YA ( 11287613 ). NFYA is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1

Protocols

Product Specific Protocols
WB protocol for NFYA antibody 12981-1-AP	Download protocol
IHC protocol for NFYA antibody 12981-1-AP	Download protocol
IF protocol for NFYA antibody 12981-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article
human,mouse	WB	J Virol Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection. Authors - Matthew S Fuller View Article
human	WB	Int J Oncol Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer. Authors - Bin Cao KD Validated View Article
rat	WB	Cell Death Dis PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells. Authors - He Liu KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NFYA Polyclonal antibody

NFYA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 12981-1-AP

WB analysis of HT-1080 using 12981-1-AP

WB analysis of K-562 using 12981-1-AP

WB analysis of HeLa using 12981-1-AP

WB analysis of Jurkat using 12981-1-AP

IHC staining of human colon cancer using 12981-1-AP

IHC staining of human prostate cancer using 12981-1-AP

IHC staining of human prostate cancer using 12981-1-AP

IHC staining of human colon cancer using 12981-1-AP

IF Staining of HepG2 using 12981-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection.

Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer.

PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells.