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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using NFYA Polyclonal antibody (12981-1-AP)

    WB analysis of HEK-293 using 12981-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using NFYA Polyclonal antibody (12981-1-AP)

    WB analysis of HT-1080 using 12981-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NFYA Polyclonal antibody (12981-1-AP)

    WB analysis of K-562 using 12981-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NFYA Polyclonal antibody (12981-1-AP)

    WB analysis of HeLa using 12981-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NFYA Polyclonal antibody (12981-1-AP)

    WB analysis of Jurkat using 12981-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NFYA Polyclonal antibody (12981-1-AP)

    IHC staining of human colon cancer using 12981-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NFYA Polyclonal antibody (12981-1-AP)

    IHC staining of human prostate cancer using 12981-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using NFYA Polyclonal antibody (12981-1-AP)

    IHC staining of human prostate cancer using 12981-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NFYA Polyclonal antibody (12981-1-AP)

    IHC staining of human colon cancer using 12981-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NFYA Polyclonal antibody (12981-1-AP)

    IF Staining of HepG2 using 12981-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFYA antibody (12981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  • Featured Product
  • KD/KO Validated

NFYA Polyclonal antibody

NFYA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12981-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NF-YA, NF YA, HAP2, CBF B, CBF A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HT-1080 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12981-1-AP targets NFYA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFYA fusion protein Ag3604 相同性解析による交差性が予測される生物種
Full Name nuclear transcription factor Y, alpha
Calculated molecular weight318aa,34 kDa; 347aa,39 kDa
Observed molecular weight 40 kDa, 46 kDa
GenBank accession numberBC039244
Gene Symbol NFYA
Gene ID (NCBI) 4800
RRIDAB_10644128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23511
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NFYA, also named as Nuclear transcription factor Y subunit alpha, is a 347 amino acid protein, which contains 1 NFYA/HAP2-type DNA-binding domain and belongs to the NFYA/HAP2 subunit family. NFYA exists the two 41- to 45-kDa isoforms of NF-YA ( 11287613 ). NFYA is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1

Protocols

Product Specific Protocols
WB protocol for NFYA antibody 12981-1-APDownload protocol
IHC protocol for NFYA antibody 12981-1-APDownload protocol
IF protocol for NFYA antibody 12981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
human,mouseWB

J Virol

Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection.

Authors - Matthew S Fuller
View Article
humanWB

Int J Oncol

Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer.

Authors - Bin Cao
  • KD Validated
View Article
ratWB

Cell Death Dis

PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells.

Authors - He Liu
  • KD Validated
View Article