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  • Featured Product
  • KD/KO Validated

NGAL Polyclonal antibody, PBS Only

NGAL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 26991-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using NGAL Polyclonal antibody (26991-1-AP)

    WB analysis of human saliva using 26991-1-AP (same clone as 26991-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NGAL Polyclonal antibody (26991-1-AP)

    WB analysis using 26991-1-AP (same clone as 26991-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using NGAL Polyclonal antibody (26991-1-AP)

    WB analysis of HT-29 using 26991-1-AP (same clone as 26991-1-PBS)

    WB result of NGAL antibody (26991-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NGAL transfected HT-29 cells. This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NGAL Polyclonal antibody (26991-1-AP)

    IHC staining of human colon cancer using 26991-1-AP (same clone as 26991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NGAL Polyclonal antibody (26991-1-AP)

    IHC staining of human colon cancer using 26991-1-AP (same clone as 26991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NGAL Polyclonal antibody (26991-1-AP)

    IHC staining of human stomach cancer using 26991-1-AP (same clone as 26991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (Lipocalin-2/NGAL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NGAL Polyclonal antibody (26991-1-AP)

    IHC staining of human stomach cancer using 26991-1-AP (same clone as 26991-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26991-1-AP (LCN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NGAL Polyclonal antibody (26991-1-AP)

    IF Staining of A431 using 26991-1-AP (same clone as 26991-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Lipocalin-2/NGAL antibody (26991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using NGAL Polyclonal antibody (26991-1-AP)

    FC experiment of A431 using 26991-1-AP (same clone as 26991-1-PBS)

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Lipocalin-2/NGAL (26991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 26991-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26991-1-PBS targets NGAL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGAL fusion protein Ag25715 相同性解析による交差性が予測される生物種
Full Name lipocalin 2
Observed molecular weight 25 kDa
Gene symbol NGAL/LCN2
Gene ID (NCBI) 3934
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Lipocalin-2 (LCN-2) is a adipocytokine also referred to as neutrophil gelatinase-associated lipocalin (NGAL). Lipocalin-2 is a circulatory protein responsible for the transportation of small and hydrophobic molecules to target organs. Lipocalin-2 is used as a biomarker for acute and chronic renal injury. It is present in a large variety of cells including neutrophil, hepatocytes, lung, bone marrow, adipose tissue, macrophages, thymus, non-neoplastic breast duct, prostate, and renal cells. Different functions have been associated with Lipocalin-2, including antibacterial, anti-inflammatory, and protection against cell and tissue stress (PMID:34463264).