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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NGDN Polyclonal antibody

NGDN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16524-1-AP

Synonyms

LCP5, EIF4E-binding protein, EIF4E binding protein, Centromere accumulated nuclear protein 1, CANu1

Validation Data Gallery

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  • Western Blot (WB) analysis of human cerebellum tissue using NGDN Polyclonal antibody (16524-1-AP)

    WB analysis of human cerebellum using 16524-1-AP

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using NGDN Polyclonal antibody (16524-1-AP)

    WB analysis of mouse testis using 16524-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NGDN Polyclonal antibody (16524-1-AP)

    WB analysis of mouse brain using 16524-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NGDN Polyclonal antibody (16524-1-AP)

    WB analysis of mouse brain using 16524-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16524-1-AP (NGDN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human epididymis tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human epididymis using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human epididymis tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human epididymis using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human epididymis using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human heart using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human heart using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human kidney using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human kidney using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human placenta using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human placenta using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human liver using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using NGDN Polyclonal antibody (16524-1-AP)

    IHC staining of human liver using 16524-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16524-1-AP (NGDN antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NGDN Polyclonal antibody (16524-1-AP)

    IF Staining of HeLa using 16524-1-AP

    Immunofluorescent analysis of Hela cells, using NGDN antibody 16524-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive WB detected inhuman cerebellum tissue, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman epididymis tissue, human heart tissue, human kidney tissue, human liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16524-1-AP targets NGDN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGDN fusion protein Ag9763 相同性解析による交差性が予測される生物種
Full Name neuroguidin, EIF4E binding protein
Calculated molecular weight 315 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC030817
Gene symbol NGDN
Gene ID (NCBI) 25983
RRIDAB_2152270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NGDN antibody 16524-1-APDownload protocol
IHC protocol for NGDN antibody 16524-1-APDownload protocol
IF protocol for NGDN antibody 16524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

A viral Sm-class RNA base-pairs with mRNAs and recruits microRNAs to inhibit apoptosis.

Authors - Carlos Gorbea
View Article