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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NGEF Polyclonal antibody

NGEF Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13271-1-AP

Synonyms

EPHEXIN, Ephexin 1, Ephexin-1, NGEF

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NGEF Polyclonal antibody (13271-1-AP)

    WB analysis using 13271-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13271-1-AP (NGEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using NGEF Polyclonal antibody (13271-1-AP)

    WB analysis of mouse testis using 13271-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13271-1-AP (NGEF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using NGEF Polyclonal antibody (13271-1-AP)

    IP experiment of mouse brain using 13271-1-AP

    IP result of anti-NGEF (IP:13271-1-AP, 4ug; Detection:13271-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using NGEF Polyclonal antibody (13271-1-AP)

    IHC staining of human brain using 13271-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NGEF Polyclonal antibody (13271-1-AP)

    IHC staining of human brain using 13271-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NGEF Polyclonal antibody (13271-1-AP)

    IHC staining of human brain using 13271-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NGEF Polyclonal antibody (13271-1-AP)

    IHC staining of human brain using 13271-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13271-1-AP (NGEF antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13271-1-AP targets NGEF in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGEF fusion protein Ag3967 相同性解析による交差性が予測される生物種
Full Name neuronal guanine nucleotide exchange factor
Calculated molecular weight 710 aa, 82 kDa
Observed molecular weight 71 kDa
GenBank accession numberBC031573
Gene symbol NGEF
Gene ID (NCBI) 25791
RRIDAB_2152381
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NGEF antibody 13271-1-APDownload protocol
IHC protocol for NGEF antibody 13271-1-APDownload protocol
IP protocol for NGEF antibody 13271-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Brain Res Bull

Electroacupuncture ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment through suppressing oxidative stress and neuroinflammation in rats

Authors - Tao Tong
View Article