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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NGEF Polyclonal antibody

NGEF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30229-1-AP

Synonyms

EPHEXIN, Ephexin 1, Ephexin-1, NGEF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NGEF Polyclonal antibody (30229-1-AP)

    WB analysis using 30229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NGEF Polyclonal antibody (30229-1-AP)

    WB analysis using 30229-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30229-1-AP (NGEF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inPC-3 cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30229-1-AP targets NGEF in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGEF fusion protein Ag30842 相同性解析による交差性が予測される生物種
Full Name neuronal guanine nucleotide exchange factor
Calculated molecular weight 710 aa, 82 kDa
Observed molecular weight71 kDa
GenBank accession numberBC031573
Gene symbol NGEF
Gene ID (NCBI) 25791
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NGEF enables guanyl-nucleotide exchange factor activity. Predicted to be involved in several processes, including activation of GTPase activity; ephrin receptor signaling pathway; and negative regulation of dendritic spine morphogenesis.

Protocols

Product Specific Protocols
WB protocol for NGEF antibody 30229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols