p75NTR Polyclonal antibody
p75NTR Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 55014-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human spleen tissue, human prostate hyperplasia tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 14 publications below |
IHC | See 5 publications below |
CoIP | See 1 publications below |
Product Information
55014-1-AP targets p75NTR in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | nerve growth factor receptor (TNFR superfamily, member 16) |
Calculated molecular weight | 45 kDa |
Observed molecular weight | 70-75 kDa |
GenBank accession number | NM_002507 |
Gene symbol | p75NTR |
Gene ID (NCBI) | 4804 |
RRID | AB_10858484 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.
Protocols
Product Specific Protocols | |
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WB protocol for p75NTR antibody 55014-1-AP | Download protocol |
IHC protocol for p75NTR antibody 55014-1-AP | Download protocol |
IP protocol for p75NTR antibody 55014-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Int J Mol Sci Direct Enhancement Effect of Hippocampal Cholinergic Neurostimulating Peptide on Cholinergic Activity in the Hippocampus | ||
Int J Mol Sci Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review | ||
Stem Cell Res Ther NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model. | ||
J Mol Med (Berl) MESP2 variants contribute to conotruncal heart defects by inhibiting cardiac neural crest cell proliferation. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Renato (Verified Customer) (02-28-2019) | Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.
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