p75NTR Polyclonal antibody

p75NTR Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 55014-1-AP

Synonyms

NGFR, Low-affinity nerve growth factor receptor p75NGR, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor, Gp80-LNGFR


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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human spleen tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55014-1-AP targets p75NTR in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name nerve growth factor receptor (TNFR superfamily, member 16)
Calculated molecular weight 45 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberNM_002507
Gene symbol p75NTR
Gene ID (NCBI) 4804
RRIDAB_10858484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NGFR, also named as TNFRSF16, Gp80-LNGFR, p75 ICD and CD271, is a low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It mediates cell survival as well as cell death of neural cells. The antibody is specific to NGFR.

Protocols

Product Specific Protocols
WB protocol for p75NTR antibody 55014-1-APDownload protocol
IHC protocol for p75NTR antibody 55014-1-APDownload protocol
IP protocol for p75NTR antibody 55014-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

J Exp Med

CXCR4 regulates Plasmodium development in mouse and human hepatocytes.

Authors - Hironori Bando
mouseWB

Int J Mol Sci

Direct Enhancement Effect of Hippocampal Cholinergic Neurostimulating Peptide on Cholinergic Activity in the Hippocampus

Authors - Yuta Madokoro
humanIHC

Int J Mol Sci

Early Clinical Outcomes of the First Commercialized Human Autologous Ex Vivo Cultivated Oral Mucosal Epithelial Cell Transplantation for Limbal Stem Cell Deficiency: Two Case Reports and Literature Review

Authors - Hiroshi Toshida
human,mouseWB,IHC

Front Oncol

LMO1 Plays an Oncogenic Role in Human Glioma Associated With NF-kB Pathway.

Authors - Lei Gao
humanWB

Stem Cell Res Ther

NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model.

Authors - Ke Wu
human

J Mol Med (Berl)

MESP2 variants contribute to conotruncal heart defects by inhibiting cardiac neural crest cell proliferation.

Authors - Erge Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Renato (Verified Customer) (02-28-2019)

Produces 3 perfectly discerning bands, but one of which clearly identifiable as p75NTR as it shows at the expected molecular weight. Used as diluent 5% BSA.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Brain tissue