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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NGFRAP1 Polyclonal antibody

NGFRAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10446-1-AP

Synonyms

NADE, HGR74, DXS6984E, Brain-expressed X-linked protein 3, BEX3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NGFRAP1 Polyclonal antibody (10446-1-AP)

    WB analysis using 10446-1-AP

    HepG2 lysates were subjected to SDS PAGE followed by western blot with 10446-1-AP (NGFRAP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using NGFRAP1 Polyclonal antibody (10446-1-AP)

    WB analysis of mouse liver using 10446-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10446-1-AP (NGFRAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NGFRAP1 Polyclonal antibody (10446-1-AP)

    IHC staining of human stomach cancer using 10446-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10446-1-AP (NGFRAP1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

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Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10446-1-AP targets NGFRAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NGFRAP1 fusion protein Ag0378 相同性解析による交差性が予測される生物種
Full Name nerve growth factor receptor (TNFRSF16) associated protein 1
Calculated molecular weight 13 kDa, 22 kDa, 44 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC003190
Gene symbol NGFRAP1
Gene ID (NCBI) 27018
RRIDAB_2152792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NGFRAP1 antibody 10446-1-APDownload protocol
IHC protocol for NGFRAP1 antibody 10446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols