Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human brain tissue, human heart tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
14885-1-AP targets NGRN in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NGRN fusion protein Ag6661 相同性解析による交差性が予測される生物種 |
| Full Name | neugrin, neurite outgrowth associated |
| Calculated molecular weight | 32 kDa |
| Observed molecular weight | 30-32 kDa |
| GenBank accession number | BC001682 |
| Gene Symbol | NGRN |
| Gene ID (NCBI) | 51335 |
| RRID | AB_2878090 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NPE2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NGRN, also named as Neugrin or FI58G, is a 291 amino acid protein, which belongs to the neugrin family. NGRN localizes in the nucleus and is expressed at high levels in heart, brain and skeletal muscle. In brain, it is mainly expressed in neurons rather than glial cells. NGRN may be involved in neuronal differentiation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NGRN antibody 14885-1-AP | Download protocol |
| IHC protocol for NGRN antibody 14885-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.
| ||
EMBO Rep A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability. | ||
J Proteomics Profiling and identification of new proteins involved in brain ischemia using MALDI-imaging-mass-spectrometry. |