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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NHE1 Polyclonal antibody

NHE1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29761-1-AP

Synonyms

SLC9A1, NHE 1, Na(+)/H(+) exchanger 1, Na(+)/H(+) antiporter, amiloride-sensitive, FLJ42224

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NHE1 Polyclonal antibody (29761-1-AP)

    WB analysis using 29761-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NHE1 Polyclonal antibody (29761-1-AP)

    WB analysis using 29761-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29761-1-AP (NHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NHE1 Polyclonal antibody (29761-1-AP)

    IHC staining of human stomach using 29761-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using NHE1 Polyclonal antibody (29761-1-AP)

    IHC staining of human stomach using 29761-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 29761-1-AP (NHE1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NHE1 Polyclonal antibody (29761-1-AP)

    IF Staining of HeLa using 29761-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using NHE1 antibody (29761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, rat brain tissue, rat heart tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29761-1-AP targets NHE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHE1 fusion protein Ag31192 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 1
Calculated molecular weight 91 kDa
Observed molecular weight90-100 kDa
GenBank accession numberNM_003047
Gene symbol NHE1
Gene ID (NCBI) 6548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NHE1, also named as SLC9A1, encodes an Na+/H+ antiporter that is a membrane-bound enzyme involved in regulating pH of vertebrate cells. NHE1 regulates a number of cell behaviors, including adhesion, shape determination, migration, and proliferation (PMID:11807182). It is inhibited by the non-specific diuretic drug amiloride and activated by growth factors, mitogens, neurotransmitters, tumor promoters, and others (PMID: 2846238). NHE1 is expressed in heart where it contributes to cardiomyocyte pH homeostasis (PMID: 12832126, PMID: 23709596).

Protocols

Product Specific Protocols
WB protocol for NHE1 antibody 29761-1-APDownload protocol
IHC protocol for NHE1 antibody 29761-1-APDownload protocol
IF protocol for NHE1 antibody 29761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Katja (Verified Customer) (06-05-2024)

test in CHO cells parental (lane 1) and NHE1 knockout CHO cells (lane 2)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: CHO
NHE1 Antibody Western Blot validation (1:2000 dilution) in CHO (Cat no:29761-1-AP)