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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NHE2 Polyclonal antibody (25105-1-AP)

    WB analysis using 25105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25105-1-AP (NHE2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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NHE2 Polyclonal antibody

NHE2 Polyclonal Antibody for WB, ELISA
Cat No. 25105-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Na(+)/H(+) exchanger 2, NHE 2, NHE2, NHE2/SLC9A2, SLC9A2, Sodium/hydrogen exchanger 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, rat brain tissue, mouse stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25105-1-AP targets NHE2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHE2 fusion protein Ag18801 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 2
Calculated molecular weight 812 aa, 92 kDa
Observed molecular weight70 kDa
GenBank accession numberBC136377
Gene Symbol NHE2/SLC9A2
Gene ID (NCBI) 6549
RRIDAB_3085771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NHE2, also known as SLC9A2, is a protein-coding gene mainly at the top of gastrointestinal epithelial cells. It mainly encodes a member of the sodium-hydrogen exchanger (NHE) protein family, which is localized to the apical membrane and is easily degraded by lysosomes, resulting in a short half-life. NHE2 is involved in sodium ion transport by exchanging intracellular hydrogen ions into external sodium ions, and helps in the regulation of cell pH and volume to eliminate acids produced by active metabolism or against adverse environmental conditions. NHE2 is widely distributed in tissues of the gastrointestinal tract, kidney, heart, testes, uterus, and adrenal glands (PMID: 37688782). The observed molecular weight of NHE2 is 70 kDa, which might be the unmodified form of NHE2 (PMID: 10444453, 10666043).

Protocols

Product Specific Protocols
WB protocol for NHE2 antibody 25105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols