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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using NHEDC2 Polyclonal antibody (24065-1-AP)

    WB analysis of human plasma using 24065-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 24065-1-AP (NHEDC2 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human brain tissue using NHEDC2 Polyclonal antibody (24065-1-AP)

    IHC staining of human brain using 24065-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24065-1-AP (NHEDC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using NHEDC2 Polyclonal antibody (24065-1-AP)

    IHC staining of human brain using 24065-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24065-1-AP (NHEDC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NHEDC2 Polyclonal antibody

NHEDC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24065-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

NHA2, NHE10, NHEDC2, SLC9B2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24065-1-AP targets NHEDC2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHEDC2 fusion protein Ag21141 相同性解析による交差性が予測される生物種
Full Name Na+/H+ exchanger domain containing 2
Calculated molecular weight 537 aa, 58 kDa
Observed molecular weight ~55 kDa
GenBank accession numberBC047447
Gene Symbol NHEDC2
Gene ID (NCBI) 133308
RRIDAB_2879423
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86UD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NHEDC2, also named as NHA2 and SLC9B2, contributes to the organellar volume homeostasis. It is required for osteoclast differentiation and bone resorption activity. NHEDC2 has two isoforms with MW 48 kDa and 58 kDa.

Protocols

Product Specific Protocols
WB protocol for NHEDC2 antibody 24065-1-APDownload protocol
IHC protocol for NHEDC2 antibody 24065-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols