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XLF Polyclonal antibody

XLF Polyclonal Antibody for WB, ELISA
Cat No. 11888-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

11888-1-AP targets XLF in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XLF fusion protein Ag2496 相同性解析による交差性が予測される生物種
Full Name nonhomologous end-joining factor 1
Calculated molecular weight 35 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC030986
Gene Symbol XLF
Gene ID (NCBI) 79840
RRIDAB_2282851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H9Q4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.

Protocols

Product Specific Protocols
WB protocol for XLF antibody 11888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Genet

Haploinsufficiency in non-homologous end joining factor 1 induces ovarian dysfunction in humans and mice.

Authors - Guoqing Li
humanWB

PLoS One

5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation.

Authors - Wei Jiang
humanWB

Int J Biochem Cell Biol

Targeting Ku86 enhances X-ray-induced radiotherapy sensitivity in serous ovarian cancer cells.

Authors - Qian Ma
humanWB

FEBS Lett

RNase L facilitates the repair of DNA double-strand breaks through the nonhomologous end-joining pathway.

Authors - Yiran Zhong
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