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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XLF Monoclonal antibody

XLF Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F8C7

Cat no : 66552-1-Ig

Synonyms

NHEJ1, Protein cernunnos, XLF, XRCC4 like factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XLF Monoclonal antibody (66552-1-Ig)

    WB analysis using 66552-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66552-1-Ig (XLF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using XLF Monoclonal antibody (66552-1-Ig)

    IHC staining of human testis using 66552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using XLF Monoclonal antibody (66552-1-Ig)

    IHC staining of human testis using 66552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66552-1-Ig (XLF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using XLF Monoclonal antibody (66552-1-Ig)

    IHC staining of human kidney using 66552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66552-1-Ig (NHEJ1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using XLF Monoclonal antibody (66552-1-Ig)

    IHC staining of human kidney using 66552-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66552-1-Ig (NHEJ1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-30000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66552-1-Ig targets XLF in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XLF fusion protein Ag2496 相同性解析による交差性が予測される生物種
Full Name nonhomologous end-joining factor 1
Calculated molecular weight 35 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC030986
Gene symbol XLF
Gene ID (NCBI) 79840
RRIDAB_2881914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

XRCC4-like factor (XLF), also known as Cernunnos and NHEJ1, is a protein encoded by the human NHEJ1 gene and an important repair factor for DNA double-strand breaks. NHEJ is a well-known mechanism for the repair of double-strand breaks (DSBs), a lethal type of DNA damage in mammalian cells. It basically utilizes a group of enzymes to take hold of the ends of a broken DNA molecule, create a bridge between them, and subsequently re-ligate the broken DNA molecule.

Protocols

Product Specific Protocols
WB protocol for XLF antibody 66552-1-IgDownload protocol
IHC protocol for XLF antibody 66552-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols