Validation Data Gallery
Tested Applications
Positive WB detected in | U-251 cells, mouse spleen tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
29416-1-AP targets NHLRC2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Reactivity | Human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NHLRC2 fusion protein Ag30159 相同性解析による交差性が予測される生物種 |
Full Name | NHL repeat containing 2 |
Calculated molecular weight | 79 kDa |
Observed molecular weight | 80 kDa |
GenBank accession number | NM_198514 |
Gene Symbol | NHLRC2 |
Gene ID (NCBI) | 374354 |
RRID | AB_2918302 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8NBF2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for NHLRC2 antibody 29416-1-AP | Download protocol |
IHC protocol for NHLRC2 antibody 29416-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |