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KD/KO Validated

NHP2 Polyclonal antibody

Name: NHP2 Polyclonal antibody
Brand: Proteintech
SKU: 15128-1-AP

NHP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15128-1-AP

Synonyms

NOLA2, NHP2P, NHP 2, HSPC286

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of SH-SY5Y using 15128-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Caco-2 using 15128-1-AP
Caco-2 cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15128-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15128-1-AP (NHP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 15128-1-AP
IP result of anti-NHP2 (IP:15128-1-AP, 4ug; Detection:15128-1-AP 1:500) with HepG2 cells lysate 3600ug.
IHC staining of human prostate cancer using 15128-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human prostate cancer using 15128-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15128-1-AP (NHP2 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of HepG2 using 15128-1-AP
Immunofluorescent analysis of HepG2 cells, using NHP2 antibody 15128-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Cat No. 15128-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SH-SY5Y cells, Caco-2 cells, HeLa cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 3 publications below
IF	See 1 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

15128-1-AP targets NHP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, yeast
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NHP2 fusion protein Ag7251 相同性解析による交差性が予測される生物種
Full Name	NHP2 ribonucleoprotein homolog (yeast)
Calculated molecular weight	17 kDa
Observed molecular weight	17-20 kDa
GenBank accession number	BC000009
Gene symbol	NHP2
Gene ID (NCBI)	55651
RRID	AB_2267290
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

Protocols

Product Specific Protocols
WB protocol for NHP2 antibody 15128-1-AP	Download protocol
IHC protocol for NHP2 antibody 15128-1-AP	Download protocol
IF protocol for NHP2 antibody 15128-1-AP	Download protocol
IP protocol for NHP2 antibody 15128-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature Structure of human telomerase holoenzyme with bound telomeric DNA. Authors - George E Ghanim View Article
human	WB	Nature Cryo-EM structure of substrate-bound human telomerase holoenzyme. Authors - Thi Hoang Duong Nguyen View Article
human	WB,IF	EMBO J NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres. Authors - Maya Raghunandan KD Validated View Article
human,yeast	WB	EMBO Rep Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis. Authors - Julia S P Mawer View Article
mouse	IHC	Biomedicines Comparative Proteomic Analysis of tPVAT during Ang II Infusion. Authors - Xiuying Liang View Article
human	WB	Mol Cell Biol SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization. Authors - D E MacNeil View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NHP2 Polyclonal antibody

NHP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of SH-SY5Y using 15128-1-AP

WB analysis of Caco-2 using 15128-1-AP

WB analysis of HeLa using 15128-1-AP

IP experiment of HepG2 using 15128-1-AP

IHC staining of human prostate cancer using 15128-1-AP

IHC staining of human prostate cancer using 15128-1-AP

IF Staining of HepG2 using 15128-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Structure of human telomerase holoenzyme with bound telomeric DNA.

Cryo-EM structure of substrate-bound human telomerase holoenzyme.

NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.

Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.