Validation Data Gallery
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Tested Applications
| Positive WB detected in | SH-SY5Y cells, Caco-2 cells, HeLa cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 13 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
15128-1-AP targets NHP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, yeast |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NHP2 fusion protein Ag7251 相同性解析による交差性が予測される生物種 |
| Full Name | NHP2 ribonucleoprotein homolog (yeast) |
| Calculated molecular weight | 17 kDa |
| Observed molecular weight | 17-20 kDa |
| GenBank accession number | BC000009 |
| Gene Symbol | NHP2 |
| Gene ID (NCBI) | 55651 |
| RRID | AB_2267290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NX24 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IHC protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IF protocol for NHP2 antibody 15128-1-AP | Download protocol |
| IP protocol for NHP2 antibody 15128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.
| ||
EMBO Rep Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis. | ||
Mol Cell Biol SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization. |