Entactin Polyclonal antibody
Entactin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13766-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | human placenta tissue, mouse heart tissue, mouse placenta tissue |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13766-1-AP targets Entactin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Entactin fusion protein Ag4749 相同性解析による交差性が予測される生物種 |
| Full Name | nidogen 1 |
| Calculated molecular weight | 1247 aa, 136 kDa |
| Observed molecular weight | 136-150 kDa |
| GenBank accession number | BC045606 |
| Gene symbol | Entactin/NID |
| Gene ID (NCBI) | 4811 |
| RRID | AB_10642564 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Entactin antibody 13766-1-AP | Download protocol |
| IHC protocol for Entactin antibody 13766-1-AP | Download protocol |
| IF protocol for Entactin antibody 13766-1-AP | Download protocol |
| IP protocol for Entactin antibody 13766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Nidogen1-enriched extracellular vesicles accelerate angiogenesis and bone regeneration by targeting Myosin-10 to regulate endothelial cell adhesion. | ||
Matrix Biol Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility. | ||
Biomaterials Stiffness assisted cell-matrix remodeling trigger 3D mechanotransduction regulatory programs |
