Nidogen 2 Polyclonal antibody
Nidogen 2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13530-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue, human placenta tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human kidney tissue, mouse skeletal muscle tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
13530-1-AP targets Nidogen 2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Nidogen 2 fusion protein Ag4424 相同性解析による交差性が予測される生物種 |
| Full Name | nidogen 2 (osteonidogen) |
| Calculated molecular weight | 1375 aa, 151 kDa |
| Observed molecular weight | 200 kDa |
| GenBank accession number | BC035608 |
| Gene symbol | NID2 |
| Gene ID (NCBI) | 22795 |
| RRID | AB_10598468 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nidogen, also referred to as entactin, represents a highly conserved protein in vertebrates and invertebrates and is found in almost all basement membranes (BM). Nidogen-1 (150 kDa) and nidogen-2 (200 kDa) are elongated molecules composed of three globular domains (G1, G2, and G3) connected by a flexible, protease-sensitive link and a rigid rod-like domain. Western blot analysis of protein extracts of heart, lung, and skeletal muscle of mice, major bands of about 200 kDa were detected, accompanied by smaller and variable bands of 160-170 kDa. (PMID: 12215539)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Nidogen 2 antibody 13530-1-AP | Download protocol |
| IHC protocol for Nidogen 2 antibody 13530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis. | ||
Int Immunopharmacol Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy |
