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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nidogen 2 Polyclonal antibody

Nidogen 2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19076-1-AP

Synonyms

NID 2, NID2, Nidogen 2, nidogen 2 (osteonidogen), Osteonidogen

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Nidogen 2 Polyclonal antibody (19076-1-AP)

    WB analysis of human placenta using 19076-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 19076-1-AP (Nidogen 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Nidogen 2 Polyclonal antibody (19076-1-AP)

    WB analysis of mouse heart using 19076-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19076-1-AP (Nidogen 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Nidogen 2 Polyclonal antibody (19076-1-AP)

    IHC staining of human placenta using 19076-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19076-1-AP (Nidogen 2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Nidogen 2 Polyclonal antibody (19076-1-AP)

    IHC staining of human placenta using 19076-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 19076-1-AP (Nidogen 2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman placenta tissue, mouse heart tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19076-1-AP targets Nidogen 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nidogen 2 fusion protein Ag4643 相同性解析による交差性が予測される生物種
Full Name nidogen 2 (osteonidogen)
Calculated molecular weight 1375 aa, 151 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC035608
Gene symbol NID2
Gene ID (NCBI) 22795
RRIDAB_2153428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Nidogen 2 antibody 19076-1-APDownload protocol
IHC protocol for Nidogen 2 antibody 19076-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols