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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NIP30 Polyclonal antibody

NIP30 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16830-1-AP

Synonyms

PA28G-interacting protein, NEFA-interacting nuclear protein NIP30, FAM192A, CDA10, CDA018

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NIP30 Polyclonal antibody (16830-1-AP)

    WB analysis of HeLa using 16830-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16830-1-AP (NIP30 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NIP30 Polyclonal antibody (16830-1-AP)

    IP experiment of HeLa using 16830-1-AP

    IP result of anti-NIP30 (IP:16830-1-AP, 4ug; Detection:16830-1-AP 1:500) with HeLa cells lysate 1080ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NIP30 Polyclonal antibody (16830-1-AP)

    IF Staining of HeLa using 16830-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16830-1-AP (NIP30 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16830-1-AP targets NIP30 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIP30 fusion protein Ag10439 相同性解析による交差性が予測される生物種
Full Name NEFA-interacting nuclear protein NIP30
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC071952
Gene symbol NIP30
Gene ID (NCBI) 80011
RRIDAB_2109703
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NIP30, also known as NEFA-interacting nuclear protein 30, FAM192A (family with sequence similarity 192, member A), is a 254 amino acid nuclear protein encoded by a gene that maps to human chromosome 16q13.

Protocols

Product Specific Protocols
WB protocol for NIP30 antibody 16830-1-APDownload protocol
IF protocol for NIP30 antibody 16830-1-APDownload protocol
IP protocol for NIP30 antibody 16830-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

The REGγ inhibitor NIP30 increases sensitivity to chemotherapy in p53-deficient tumor cells.

Authors - Xiao Gao
View Article
humanWB

Retrovirology

Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses.

Authors - Doueiri Rami R
View Article