NIPBL Polyclonal antibody
NIPBL Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 18792-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IP | See 1 publications below |
| ChIP | See 2 publications below |
Product Information
18792-1-AP targets NIPBL in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NIPBL fusion protein Ag5663 相同性解析による交差性が予測される生物種 |
| Full Name | Nipped-B homolog (Drosophila) |
| Calculated molecular weight | 2804 aa, 316 kDa |
| Observed molecular weight | 300 kDa |
| GenBank accession number | BC033847 |
| Gene symbol | NIPBL |
| Gene ID (NCBI) | 25836 |
| RRID | AB_2151288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NIPBL antibody 18792-1-AP | Download protocol |
| IF protocol for NIPBL antibody 18792-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. | ||
Nat Immunol BRWD1 orchestrates small pre-B cell chromatin topology by converting static to dynamic cohesin | ||
Nucleic Acids Res BRD2 promotes antibody class switch recombination by facilitating DNA repair in collaboration with NIPBL |
