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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    WB analysis of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    WB analysis of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18792-1-AP (NIPBL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NIPBL Polyclonal antibody (18792-1-AP)

    IF Staining of HeLa using 18792-1-AP (same clone as 18792-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 18792-1-AP (NIPBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18792-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18792-1-PBS targets NIPBL in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NIPBL fusion protein Ag5663 相同性解析による交差性が予測される生物種
Full Name Nipped-B homolog (Drosophila)
Calculated molecular weight 2804 aa, 316 kDa
Observed molecular weight 300 kDa
GenBank accession numberBC033847
Gene Symbol NIPBL
Gene ID (NCBI) 25836
RRIDAB_2151288
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6KC79
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NIPBL, also named as Nipped-B-like protein, is a 28-4 amino acid protein, which Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains and belongs to the SCC2/Nipped-B family. NIPBL probably plays a structural role in chromatin and Involve in sister chromatid cohesion, possibly by interacting with the cohesin complex. endogenous full length NIPBL which is estimated to be about 300kDa led us to believe that the NIPBL protein was deteriorated or lost by the standard protein extraction and blotting techniques.